profile image for Dr Scott Wildman PhD

Dr Scott Wildman PhD

Senior Lecturer

Medway School of Pharmacy

 

Scott graduated with a BSc (Hons) in Pharmacology from The University of Sunderland in 1995 and was awarded a PhD in Neuroscience in 1999 from UCL (Department of Anatomy and Developmental Biology; supervised by Brian King and Geoffrey Burnstock).

Following his PhD, he was a post-doctoral researcher in the laboratory of Nick Franks at Imperial College London, then the laboratories of Robert Unwin at UCL and Mike Sutters at Johns Hopkins University (Maryland, USA). In 2004, Scott was awarded a 3-year British Heart Foundation Research Fellowship to conduct independent research in the Department of Physiology, UCL.

Subsequently Scott was appointed as a Lecturer, then Senior Lecturer, in Physiology and Pharmacology at the Royal Veterinary College (2007-2011) and a Senior Lecturer in Pharmaceutical Sciences at London Metropolitan University (2011).  Scott joined Medway School of Pharmacy (MSoP) as a Senior Lecturer in Biological Sciences in January 2012.  He also holds an Honorary Senior Lectureship in the UCL Division of Medicine Centre for Nephrology, UCL (awarded 2007). In 2013, Scott served as the School's Director of Learning and Teaching (2013-2016). He is currently the School's Director of Research, and the Centre Director for the Centre for Higher and Degree Apprenticeships (University of Kent).

Scott's research drives advances in the field, and he is considered a world-class researcher in urinary system physiology. During his career he have contributed to 41 high quality publications and reviews, with >1000 citations and an h-index of 23. Scott has presented work at conferences all over the world, and usually upon invitation. To date, he has attracted ~£1.9 million in research revenue (excluding internal grants). Scott's work is also creating impact and has altered clinical practice (and as such his research constituted one of Medway School of Pharmacy’s impact case submissions in REF2014).

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My research focus is urinary system physiology (i. the nature and regulation of epithelial transport processes along the nephron, which has been extended to include ii. epithelial cell function in the urinary bladder, and iii. the control of vasa recta capillary diameter by renal pericytes); a poorly represented area of basic and applied research, especially in the UK. I jointly lead a laboratory, the Urinary System Physiology Unit, which focuses on the local regulation of renal tubular transport mechanisms, and the regulation of the renal medullary blood flow.

i. My main research interest has been, and continues to be, renal epithelial cell transport function, its regulation and relationship to renal tubular cell disorders.  My research is basic, yet closely linked to clinical research, one informing the other.  My early work was on the regulation of tubular transport mechanisms by paracrine and autocrine factors and how they affect electrolyte and fluid transport.  I investigated the possible sources, actions and roles of extracellular ATP, and as a result I have played a significant part in defining the renal ATP-activated P2 receptor system in normal physiology and pathophysiology.  I went on to specifically investigate modulation of renal function from the tubule lumen, believing that renal tubular fluid composition, made up of filtered or secreted factors, may regulate or disturb tubular transport function.  To do so I developed an existing technique for making electrophysiological recordings from the apical membrane of tubular cells in situ (i.e. the isolated split-open renal tubule technique); we are currently the only research group in the UK performing this technique, and one of a few worldwide.  Using this technique I identified a luminal sodium sensor in the renal collecting duct that may locally regulate sodium reabsorption and therefore arterial blood pressure.  Consequently, we are now investigating the physiological significance of the sensor using a knock-out mouse model; and have increased our repertoire of experimental techniques to include recording from isolated perfused tubules.

ii. More recently I have extended my research interests into bladder epithelial cell function, its regulation and relationship to bladder disorders, including overactive bladder (OAB).  We are investigating the effects of bacterial colonisation of urothelial cells on paracrine and autocrine nucleotide signalling; which is especially prevalent in renal transplant recipients and may determine graft survival. 

iii. I am also interested in the role of specialised smooth-muscle-like cells, pericytes, in the regulation of the renal microvasculature.  We have developed a novel kidney slice model to investigate the role of pericytes in the regulation of in situ vasa recta capillary diameter.  Since the inner medulla is relatively inaccessible in vivo the slice model provides an ideal environment in which to characterise endogenous modulators of vasa recta pericyte contractility.  Using this slice model we are also investigating tubulovascular cross-talk, which we believe is critical in coordinating tubular function with medullary blood flow.

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Research papers (Scientific)

1. Is chronic urinary infection a cause of overactive bladder? Balachandran AA, Wildman SS, Strutt M, Duckett J. Eur J Obstet Gynecol Reprod Biol. 2016 Mar 24;201:108-112. doi: 10.1016/j.ejogrb.2016.03.024. [Epub ahead of print]

2.  Kennedy-Lydon, Teresa and Crawford, Carol and Wildman, Scott S.P. and Peppiatt-Wildman, Claire M. (2015) Nonsteroidal anti-inflammatory drugs alter vasa recta diameter via pericytes. American Journal of Physiology - Renal Physiology, 309 (7). F648-F657. ISSN 1931-857X.  http://kar.kent.ac.uk/53101/

3.  Gill, Kiren and Horsley, Harry and Kupelian, Anthony S and Baio, Gianluca and De Iorio, Maria and Sathiananamoorthy, Sanchutha and Khasriya, Rajvinder and Rohn, Jennifer L and Wildman, Scott S.P. and Malone-Lee, James (2015) Urinary ATP as an indicator of infection and inflammation of the urinary tract in patients with lower urinary tract symptoms. BMC Urology, 15 (1). p. 7. ISSN 1471-2490.  http://kar.kent.ac.uk/53100/

4.  Kelley, Stephen P. and Walsh, Jacqueline and Kelly, Mark and Mudhar, S and Adel-Aziz, M and Barrett, Iain and Wildman, Scott S.P. (2014) Inhibition of native 5-HT3 receptor-evoked contractions in guinea pig and mouse ileum by antimalarial drugs. European Jounal of Pharmacology, 738 . pp. 186-191. ISSN 0014-2999.  http://kar.kent.ac.uk/46527/

5.  Kelley, Stephen P. and Courtniege, Holly and Birch, Rebecca E. and Contreras-Sanz, Alberto and Kelly, Mark and Durodie, Jerome and Peppiatt-Wildman, Claire M. and Farmer, Chris and Delaney, Michael and Malone-Lee, James and Harber, Mark and Wildman, Scott S.P. (2014) Urinary ATP and visualization of intracellular bacteria: a superior diagnostic marker for recurrent UTI in renal transplant recipients? Springerplus, 3 . p. 1. ISSN 2193-1801.  http://kar.kent.ac.uk/46531/

6.  Crawford, C. and Wildman, Scott S.P. and Kelly, M. C. and Kennedy-Lydon, T. M. and Peppiatt-Wildman, Claire M. (2013) Sympathetic nerve-derived ATP regulates renal medullary vasa recta diameter via pericyte cells: a role for regulating medullary blood flow? Frontiers in Physiology, 4 . pp. 1-8. ISSN 1664-042X.  http://kar.kent.ac.uk/53092/

7.  Zerpa, H., Crawford, C., Knight, G. E., Fordham, A., Janska, S. E., Peppiatt-Wildman, C. M., Elliott, J., Burnstock, G., Wildman, S. S. P. (2013) Extracellular ATP signalling in equine digital vessels.  E J Pharmacol., 702, 242-249.

8.  Crawford, C. and Kennedy-Lydon, T. and Sprott, C. and Desai, T. and Sawbridge, L. and Munday, J. and Unwin, R.J. and Wildman, Scott S.P. and Peppiatt-Wildman, Claire M. (2012) An Intact Kidney Slice Model to Investigate Vasa Recta Properties and Function in situ. Nephron Physiology, 120 (3). pp. 17-31. ISSN 1660-2137.  http://kar.kent.ac.uk/53061/

9.  Contreras-Sanz, Alberto and Scott-Ward, Toby S. and Gill, Hardyal S. and Jacoby, Jennifer C. and Birch, Rebecca E. and Malone-Lee, James and Taylor, Kevin M. G. and Peppiatt-Wildman, Claire M. and Wildman, Scott S. P. (2012) Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC. Purinergic Signalling, 8 (4). pp. 741-751. ISSN 1573-9538.  http://kar.kent.ac.uk/53063/

10.  Crawford, C and Kennedy-Lydon, T M and Callaghan, H and Sprott, C and Simmons, R L and Sawbridge, L and Syme, H M and Unwin, Robert J and Wildman, Scott S.P. and Peppiatt-Wildman, Claire M. (2011) Extracellular nucleotides affect pericyte-mediated regulation of rat in situ vasa recta diameter. Acta physiologica (Oxford, England), 202 (3). pp. 241-51. ISSN 1748-1716.  http://kar.kent.ac.uk/53062/

11.  Cesaro, Annabelle and Brest, Patrick and Hofman, Véronique and Hebuterne, Xavier and Wildman, Scott S.P. and Ferrua, Bernard and Marchetti, Sandrine and Doglio, Alain and Vouret-Craviari, Valérie and Galland, Franck and Naquet, Philippe and Mograbi, Baharia and Unwin, Robert and Hofman, Paul (2010) Amplification loop of the inflammatory process is induced by P2X7R activation in intestinal epithelial cells in response to neutrophil transepithelial migration. American Journal of Physiology: Gastrointestinal and Liver Physiology, 299 (1). G32-G42. ISSN 0193-1857.  http://kar.kent.ac.uk/53060/

12.  Hernández, Medardo and Knight, Gillian E. and Wildman, Scott S. P. and Burnstock, Geoffrey (2009) Role of ATP and related purines in inhibitory neurotransmission to the pig urinary bladder neck. British journal of pharmacology, 157 (8). pp. 1463-1473. ISSN 0007-1188.  http://kar.kent.ac.uk/53058/

13.  Wildman, Scott S.P. and Boone, Michelle and Peppiatt-Wildman, Claire M. and Contreras-Sanz, Alberto and King, Brian F. and Shirley, David G. and Deen, Peter M. T. and Unwin, Robert J. (2009) Nucleotides downregulate aquaporin 2 via activation of apical P2 receptors. Journal of the American Society of Nephrology, 20 (7). pp. 1480-1490. ISSN 1046-6673.  http://kar.kent.ac.uk/53056/

14.  Wildman, Scott S.P. and Marks, Joanne and Turner, Clare M. and Yew-Booth, Liang and Peppiatt-Wildman, Claire M. and King, Brian F. and Shirley, David G. and Wang, WenHui and Unwin, Robert J. (2008) Sodium-dependent regulation of renal amiloride-sensitive currents by apical P2 receptors. Journal of the American Society of Nephrology, 19 (4). pp. 731-742. ISSN 1046-6673.  http://kar.kent.ac.uk/53052/

Review papers (Scientific)

1.  Dunn, Kadeshia and Peppiatt-Wildman, Claire M. and Kelley, Stephen P. and Wildman, Scott S.P. (2014) Current Perspective on the Location and Function of Gamma-Aminobutyric Acid (GABA) and its Metabolic Partners in the Kidney. Journal of Nephrology and Urology Research, 2 . pp. 47-57. ISSN 2310-984X.  http://kar.kent.ac.uk/46569/

2.  Birch, R. E. and Schwiebert, E. M. and Peppiatt-Wildman, Claire M. and Wildman, S. S. (2013) Emerging key roles for P2X receptors in the kidney. Frontiers in Physiology, 4. pp. 1-8. ISSN 1664-042X.  http://kar.kent.ac.uk/53088/

3.  Wildman, S.S., Kelly, M.C., Leech, H.K., Duckett, J.R.A., Stevens, P.E., & Peppiatt-Wildman, C.M. (2013) Embracing translation: avant-garde urinary system research in Kent.  Science Omega Review UK, Issue 2, 1-3.

4.  Craigie, Eilidh and Birch, Rebecca E and Unwin, Robert J and Wildman, Scott S (2013) The relationship between P2X4 and P2X7: a physiologically important interaction? Frontiers in physiology, 4. p. 216. ISSN 1664-042X.  http://kar.kent.ac.uk/53086/

5.  Harber, M., Malone-Lee, J. & Wildman, S. (2012) Asymptomatic bacteriuria and urinary tract infection in renal transplantation.  Brit J Renal Med 17, 4-7.

6.  Kennedy-Lydon, T. M. and Crawford, C. and Wildman, Scott S.P. and Peppiatt-Wildman, Claire M. (2012) Renal pericytes: regulators of medullary blood flow. Acta Physiologica, 207 (2). pp. 212-225. ISSN 1748-1708.  http://kar.kent.ac.uk/53102/

7.  Wildman, Scott S.P. and Kang, Esther S-K and King, Brian F. (2009) ENaC, renal sodium excretion and extracellular ATP. Purinergic Signalling, 5 (4). pp. 481-489. ISSN 1573-9538.  http://kar.kent.ac.uk/53055/

8.  Wildman, Scott S. P. and King, Brian F. (2008) P2X receptors: epithelial ion channels and regulators of salt and water transport. Nephron Physiology, 108 (3). p60-67. ISSN 1660-8151.  http://kar.kent.ac.uk/53053/

 

  • Pre 2008

    • Greenwood D, Jagger D J, Huang LC, Hoya N, Thorne PR, Wildman SS, King BF, Pak K, Ryan AF, Housley GD (2007). P2X2/3 receptor signaling inhibits BDNF-mediated spiral ganglion neuron development in the neonatal rat cochlea. Development 134: 1407-1417.

    • Wildman SS, Marks J, Churchill LJ, Peppiatt CM, Chraibi A, Shirley DG, Horisberger JD, King BF, Unwin RJ (2005). Regulatory interdependence of cloned epithelial Na+ channels and P2X receptors. J Am Soc Nephrol 16: 2586-2597.

    • Plested AJ, Wildman SS, Lieb WR, Franks NP (2004). Determinants of the sensitivity of AMPA receptors to xenon. Anaesthesiology 100: 347-358.

    • Wildman SS, Unwin RJ, King BF (2003). Extended pharmacological profiles of rat P2Y2 and rat P2Y4 receptors and their sensitivity to extracellular H+ and Zn2+ ions. Br J Pharmacol 140: 1177-1186.

    • Wildman SS, Hooper KM, Turner CM, Sham JS, Lakatta EG, King BF, Unwin RJ, Sutters M (2003). The isolated polycystin-1 cytoplasmic COOH terminus prolongs ATP-stimulated Cl- conductance through increased Ca2+ entry. Am J Physiol Renal Physiol 285: F1168-1178.

    • Wildman SS, Brown SG, Rahman M, Noel CA, Churchill L, Burnstock G, Unwin RJ, King BF (2002). Sensitization by extracellular Ca2+ of rat P2X5 receptor and its pharmacological properties compared with rat P2X1. Mol Pharmacol 62: 957-966.
      King BF, Townsend-Nicholson A, Wildman SS, Thomas T, Spyer KM, Burnstock G (2000). Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes. J Neurosci 20: 4871-4877.

    • Wildman SS, King BF, Burnstock G (1999). Modulation of ATP-responses at recombinant rP2X4 receptors by extracellular pH and zinc. Br J Pharmacol 126: 762-768.

    • Wildman SS, King BF, Burnstock G (1999). Modulatory activity of extracellular H+ and Zn2+ on ATP-responses at rP2X1 and rP2X3 receptors. Br J Pharmacol 128: 486-492.

    • Wildman SS, Brown SG, King BF, Burnstock G (1999). Selectivity of diadenosine polyphosphates for rat P2X receptor subunits. Eur J Pharmacol 367: 119-123.

    • Townsend-Nicholson A, King BF, Wildman SS, & Burnstock G (1999). Molecular cloning, functional characterization and possible cooperativity between the murine P2X4 and P2X4a receptors. Brain Res Mol Brain Res 64, 246-254.

    • Jacobson KA, Hoffmann C, Kim YC, Camaioni E, Nandanan E, Jang SY, Guo DP, Ji XD, Von Kugelgen I, Moro S, Ziganshin AU, Rychkov A, King BF, Brown SG, Wildman SS, Burnstock G, Boyer JL, Mohanram A, Harden TK (1999). Molecular recognition in P2 receptors: ligand development aided by molecular modeling and mutagenesis. Prog Brain Res 120: 119-132.

    • Kim KY, Camaioni E, Ziganshin AU, Ji X, King BF, Wildman SS et al (1998). Synthesis and structure relationships of pyridoxal-6-azoaryl-5'-phosphate and phosphonate derivatives as P2 receptor antagonists. Drug Dev Res 45: 52-66.

    • Wildman SS, King BF, Burnstock G (1998). Zn2+ modulation of ATP-responses at recombinant P2X2 receptors and its dependence on extracellular pH. Br J Pharmacol 123: 1214-1220.

    • King BF, Wildman SS, Townsend-Nicholson A, Burnstock G (1998). Antagonism of an adenosine/ATP receptor in follicular Xenopus oocytes. J Pharmacol Exp Ther 285: 1005-1011.

    • Jacobson KA, Kim YC, Wildman SS, Mohanram A, Harden TK, Boyer JL, King BF, Burnstock G (1998). A pyridoxine cyclic phosphate and its 6-azoaryl derivative selectively potentiate and antagonize activation of P2X1 receptors. J Med Chem 41: 2201-2206.

    • Bogdanov YD, Wildman SS, Clements MP, King BF, Burnstock G (1998). Molecular cloning and characterization of rat P2Y4 nucleotide receptor. Br J Pharmacol 124: 428-430.

    • Wildman SS, King BF, Burnstock G (1997). Potentiation of ATP-responses at a recombinant P2X2 receptor by neurotransmitters and related substances. Br J Pharmacol 120, 221-224.

    • King BF, Wildman SS, Ziganshina LE, Pintor J, Burnstock G (1997). Effects of extracellular pH on agonism and antagonism at a recombinant P2X2 receptor. Br J Pharmacol 121: 1445-1453.

     

Book Chapters

1.  Kishore, Bellamkonda and Unwin, Robert and Vallon, Volker and Prætorius, Helle and Wildman, Scott S.P. (2013) Extracellular Nucleotides in the Regulation of Kidney Functions. In: Extracellular Nucleotides in the Regulation of Kidney Functions. Frontiers Media SA.  http://kar.kent.ac.uk/53264/

2.  Alpern, Robert and Caplan, Michael and Moe, Orson and Wildman, Scott S.P. (2012) Seldin and Giebisch's The Kidney, 5th Edition, Physiology & Pathophysiology. In: Seldin and Giebisch's The Kidney (Fifth Edition) Physiology & Pathophysiology 1-2. Elsevier Inc. ISBN 978-0-12-381462-3.  http://kar.kent.ac.uk/53109/

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Last Updated 08/01/2018