profile image for Dr Claire Peppiatt-Wildman

Dr Claire Peppiatt-Wildman

Senior Lecturer in Biological Sciences

Medway School of Pharmacy

 

Claire graduated with a BSc (Hons) in Medical Biochemistry from Royal Holloway University of London in 1999 and was awarded a PhD in Cellular Physiology in 2002 from The University of Cambridge (Babraham Institute; supervised by Dr Martin Bootman and Professor Sir Michael Berridge).

Following her PhD, she was a post-doctoral researcher in the laboratory of Professor David Attwell at University College London, then the laboratories of Professor William Large at St Georges University of London. In 2007, Claire was awarded a 5-year Medical Research Council funded Career Development Award to conduct independent research in the Department of Physiology, UCL (Centre for Nephrology at The Royal Free Hospital) and then at the Royal Veterinary College (Department of Veterinary Basic Sciences).

Subsequently Claire joined Medway School of Pharmacy (MSoP) and was appointed as a Lecturer, then Senior Lecturer in Biological Sciences. Claire also holds an Honorary Lectureship in the UCL Division of Medicine, Centre for Nephrology, UCL (awarded 2010).

Throughout Claire’s career she has notably been rewarded with a number of high impact-factor peer-reviewed research publications (Nature), invitations to lecture in the UK and abroad, requests for review articles, and a Renal Research Recognition award by The American Physiological Society (2009).

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Claire’s main research focuses on the role of smooth muscle-like pericyte cells in regulating the microcirculation. She utilises imaging techniques (i.e multiphoton /confocal microscopy and video imaging techniques to monitor real time changes in vessel diameter in live tissues), luminescence and real-time fluorescence measurements (in order to quantify ATP, calcium and nitric oxide levels in the vasculature of live tissue) and electrophysiology techniques (to characterise membrane receptors, ion channels and second messengers involved in regulation of pericyte contractility). Claire’s group, part of the the Urinary System Physiology Unit which she jointly leads, is one of the only groups in the UK investigating regulation of renal microvessels in situ and her group have pioneered the use of a live kidney slice model in renal research.

In addition to these core research projects Claire runs several other projects focusing on improving our understanding of kidney function in health and disease

  • In collaboration with Andrew Hall and Professor Robert Unwin (Centre for Nephrology [CfN], UCL) Claire has developed an advanced imaging technique (multiphoton imaging) that is utilised to investigate tubular and vascular function in the isolated perfused kidney (IPK). This work has been published in J Am Soc Nephrol  ‘Multiphoton imaging of the isolated perfused rat kidney’.
  • In collaboration with Professor Mervyn Singer (Wolfson Institute, UCL) we are investigating renal blood flow regulation at the macroscopic level using Laser Doppler Flowmetry.
  • In collaboration with Dr Scott Wildman we utilise electrophysiology techniques in combination with isolated perfused tubules to investigate renal epithelial cell transport functions and their relationship to renal tubular cell disorders.

Existing research projects are listed below:

  • Pericyte-mediated intra-renal blood flow regulation: from brain to kidney (funded by the MRC £1.1 million).
  • Elucidating mechanisms of drug toxicity in the kidney using novel advanced imaging techniques (funded by The St Peters Trust, £75, 000).
  • Components of the innate immune system are mediators of tissue ischemia by altering mitochondrial function and regulating microvessel tone in acute kidney injury (funded by Pfizer £528, 000).
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  • Wildman, S.S., Kelly, M.C., Leech, H.K., Duckett, J.R.A., Stevens, P.E., & Peppiatt-Wildman, C.M. (2013) Embracing translation: avant-garde urinary system research in Kent. Science Omega Review UK, Issue 2, in press

  • Zerpa HA, Crawford C, Knight GE, Fordham AF, Janska SE, Peppiatt-Wildman CM, Elliott J, Burnstock G, Wildman SS. Extracellular ATP signaling in equine digital blood vessels. Eur J Pharmacol. 2013 Jan 29. (Epub ahead of print)

  • Kennedy-Lydon, Crawford C, Peppiatt-Wildman CM. (2012) Renal pericytes: regulators of medullary blood flow. Acta Physiological.

  • Peppiatt-Wildman CM, Crawford C, Hall AM. (2012) Fluorescence imaging of intracellular calcium signals in intact kidney tissue. Accepted Nephron Experimental.

  • Crawford C, Kennedy-Lydon T, Sprott C, Desai T, Sawbridge L, Munday J, Unwin RJ, Wildman SS, Peppiatt-Wildman CM. (2012) An Intact Kidney Slice Model to Investigate Vasa Recta Properties and Function in situ. Nephron Physiol. 120(3):p17-31. Epub 2012 Jul 20.

  • Contreras-Sanz A, Scott-Ward TS, Gill HS, Jacoby JC, Birch RE, Malone-Lee J, Taylor KM, Peppiatt-Wildman CM, Wildman SS. (2012) Simultaneous quantification of 12 different nucleotides and nucleosides released from renal epithelium and in human urine samples using ion-pair reversed-phase HPLC. Purinergic Signal. 2012 Jun 16. [Epub ahead of print]

  • Crawford C, Kennedy-Lydon TM, Callaghan H, Sprott C, Simmons RL, Sawbridge L, Syme HM, Unwin RJ, Wildman SS, Peppiatt-Wildman CM. (2011) Extracellular nucleotides affect pericyte-mediated regulation of rat in situ vasa recta diameter. Acta Physiol (Oxf).Jul;202(3):241-51.

  • Howarth C, Peppiatt-Wildman CM, Attwell D. (2010) The energy use associated with neural computation in the cerebellum. J Cereb Blood Flow Metab. 30(2) 403-14

  • Wildman SS, Boone M, Peppiatt-Wildman CM, Contreras-Sanz A, King BF, Shirley DG, Deen PM, Unwin RJ. (2009) Nucleotides downregulate aquaporin 2 via activation of apical P2 receptors. J Am Soc Nephrol. Jul;20(7):1480-90

  • SN Saleh, AP Albert, CM Peppiatt-Wildman, WA Large (2007) Diverse properties of store-operated TRPC channels activated by protein kinase C in vascular myocytes. J Physiol. 15;586(10):2463-76.

  • SSP Wildman, J Marks, CM Turner, L Yew-Booth, CM Peppiatt-Wildman, BF King, DG Shirley, W-H Wang, RJ Unwin (2008) Sodium-dependent regulation of renal amiloride-sensitive currents by apical P2 receptors. J Am Soc Nephrol. 19(4):731-42.

  • AP Albert, SN Saleh, CM Peppiatt-Wildman, WA Large. (2007) Multiple activation mechanisms of store-operated channels in smooth muscle cells. Invited Topical Review for J Physiol, 15;583(Pt 1):25-36.

  • CM Peppiatt-Wildman, AP Albert, SN Saleh, WA Large (2007) Endothelin-1 activates a Ca2+-permeable cation channel with TRPC3 and TRPC7 properties in rabbit coronary artery myocytes. J Physiol, 580(Pt 3):755-64.

  • CM Peppiatt, C Howarth, P Mobbs, D Attwell (2006) Bidirectional control of CNS capillary diameter by pericytes. Nature, 443(7112):700-4.

  • SN Saleh, AP Albert, CM Peppiatt, WA Large (2006) Angiotensin II activates two cation conductances with distinct TRPC1 and TRPC6 channel properties in rabbit mesenteric artery myocytes. J Physiol, 577(Pt 2):479-95.

  • SS Wildman, J Marks, LJ Churchill, CM Peppiatt, A Chraibi, DG Shirley, JD Horisberger, BF King, RJ Unwin (2005) Regulatory Interdependence of Cloned Epithelial Na+ Channels and P2X Receptors. J Am Soc Nephrol, 16(9):2586-97.

  • C Peppiatt and D Attwell (2004) Neurobiology: feeding the brain. Nature, 431(7005):137-8.

  • CM Peppiatt, AM Holmes, JT Seo, MD Bootman, TJ Collins, F Macdonald and HL Roderick (2004) Pharmacologically and mechanistically distinct store-operated and archidonate-acid activated calcium influx pathways in HeLa cells. Biochemical Journal, 381(Pt 3):929-39.

  • CM Peppiatt, TJ Collins, L Mackenzie, SJ Conway, AB Holmes, MD Bootman, MJ Berridge, JT Seo and HL Roderick. (2003) 2-Aminoethoxydiphenyl borate (2-APB) antagonises inositol 1,4,5-trisphosphate-induced calcium release, inhibits calcium pumps and has a use-dependent and slowly reversible action on store-operated calcium entry channels. Cell Calcium, 34(1):97-108.

  • MD Bootman, TJ Collins, L Mackenzie, HL Roderick, MJ Berridge, CM Peppiatt (2002) 2-aminoethoxydiphenyl borate (2-APB) is a reliable blocker of store-operated Ca2+ entry but an inconsistent inhibitor of InsP3-induced Ca2+ release. FASEB J.,16(10):1145-50.

  • MD Bootman, TJ Collins, CM Peppiatt, LS Prothero, L Mackenzie, P De Smet, M Travers, SC Tovey, JT Seo, MJ Berridge, F Ciccolini, P Lipp 2001 Calcium signalling - an overview. Semin Cell Dev Biol., 12(1):3-10. Review.

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Last Updated 11/04/2017